Specific association of simian virus 40 tumor antigen with simian virus 40 chromatin
نویسندگان
چکیده
منابع مشابه
Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
We have studied the binding of the tumor antigen (T-antigen) of simian virus 40 to simian virus 40 chromatin (minichromosomes). The minichromosomes isolated from infected cells by a modification of standard techniques were relatively free of contaminating RNA and cellular DNA and had a ratio (by weight) of protein to DNA of approximately 1; their DNA was 50 to 60% digestible to an acid-soluble ...
متن کاملAssociation of simian virus 40 T antigen with simian virus 40 nucleoprotein complexes.
Viral nucleoprotein complexes were extracted from the nuclei of simian virus 40 (SV40)-infected TC7 cells by low-salt treatment in the absence of detergent, followed by sedimentation on neutral sucrose gradients. Two forms of SV40 nucleoprotein complexes, those containing SV40 replicative intermediate DNA and those containing SV40 (I) DNA, were separated from one another and were found to have ...
متن کاملStimulation of simian virus 40 late gene expression by simian virus 40 tumor antigen.
The early simian virus 40 (SV40) gene product, large tumor (T) antigen, is responsible for the initiation of viral DNA replication and the autoregulation of early gene expression through direct protein-DNA interactions. We investigated the role of T antigen in late viral gene expression, independent of its function in amplifying templates through DNA replication. SV40 DNA was transfected into B...
متن کامل"Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.
Simian virus 40 (SV40) induces tumor (T)-antigen formation, chromatin replication, and mitosis in primary mouse kidney cells arrested in G0 phase of the mitotic cycle. The temporal and quantitative relation between these early virus-specific reactions led to the hypothesis that the early SV40 mRNA contains information necessary for T-antigen formation and induction of cellular DNA synthesis. To...
متن کاملSingle strand DNA binding of simian virus 40 tumor antigen.
Simian virus 40 T antigen binds to both single and double strand DNA. The single and double strand DNA binding activity of crude T antigen preparations was evaluated by chromatography of the antigen on DNA-cellulose columns. Crude T antigen was retained on both native and denatured DNA-cellulos columns and was eluted from both columns under similar conditions. The interaction of highly purified...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1980
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.33.1.78-87.1980